| Date | À domicile | A l'extérieur | Résultat |
|---|---|---|---|
| 28/10/25 19:00 | CF Ourense | Real Oviedo | 4-2a.Prlg |
| 28/10/25 19:00 | Atletico Tordesillas | Burgos CF | 1-2a.Prlg |
| 28/10/25 19:00 | CE Constància | FC Girona | 2-3a.Prlg |
| 28/10/25 19:30 | SD Logroñés | Racing Santander | 0-4 |
| 28/10/25 19:30 | UP Langreo | Racing de Ferrol | 3-4a.TAB |
| 28/10/25 19:30 | UCAM Murcia | FC Cadiz | 1-3 |
| 28/10/25 20:00 | CD Guadalajara | CP Cacereño | 2-1 |
| 28/10/25 20:00 | CF Rayo Majadahonda | CF Talavera de la Reina | 1-4 |
| 28/10/25 20:00 | UD San Fernando | Albacete Balompie | 0-3 |
| 28/10/25 20:00 | Inter de Valdemoro | FC Getafe | 0-11 |
| 28/10/25 20:00 | CD Roda | Granada CF | 1-5 |
| 28/10/25 20:00 | CD Tropezon | CyD Leonesa | 1-3 |
| 28/10/25 20:00 | UD Maracena | Valence CF | 0-5 |
| 28/10/25 20:30 | Utebo | SD Huesca | 0-3 |
| 28/10/25 20:30 | RSD Alcalá | CD Tenerife | 0-4 |
| 28/10/25 21:00 | CD Extremadura | UD Las Palmas | 3-1 |
| 28/10/25 21:00 | SD Negreira | Real Sociedad | 0-3 |
| 28/10/25 21:00 | CD Toledo | FC Séville | 1-4 |
| 29/10/25 18:00 | UD Poblense | CD Sabadell | 1-2a.Prlg |
| 29/10/25 19:00 | CD Ebro | SD Tarazona | 5-4a.TAB |
| 29/10/25 19:00 | Union Mutilvera | Real Saragosse | 1-3 |
| 29/10/25 19:00 | CD Sant Jordi | CA Osasuna | 0-5 |
| 29/10/25 19:00 | Los Garres | FC Elche | 0-4 |
| 29/10/25 19:30 | La Unión Atlético CF | AD Ceuta FC | 0-2 |
| 29/10/25 20:00 | CD Artistico Navalcarnero | Merida AD | 2-1 |
| 29/10/25 20:00 | CD Azuaga | CD Leganes | 1-4 |
| 29/10/25 20:00 | Náxara CD | SD Eibar | 2-4 |
| 29/10/25 20:00 | Atletico Astorga | CD Mirandes | 4-5a.TAB |
| 29/10/25 20:00 | CD Numancia | Arenas Club de Getxo | 3-0 |
| 29/10/25 20:00 | Lorca Deportiva CF | UD Almeria | 0-2 |
| 29/10/25 20:00 | UE Sant Andreu | CD Teruel | 2-1a.Prlg |
| 29/10/25 20:00 | Caudal Deportivo | Sporting Gijon | 0-1 |
| 29/10/25 20:00 | Atlètic Sant Just | RCD Majorque | 0-2 |
| 29/10/25 20:00 | Torrent CF | Juventud Torremolinos CF | 3-1 |
| 29/10/25 20:00 | CD Cieza | Cordoba CF | 1-0 |
| 29/10/25 20:15 | Club Portugalete | Real Valladolid | 1-0 |
| 29/10/25 20:30 | CF Reus Deportiu | CE Europa | 6-5a.TAB |
| 29/10/25 20:30 | UD Ourense | CF Pontevedra | 1-4a.TAB |
| 29/10/25 20:30 | Puente Genil CF | FC Cartagena | 0-1 |
| 29/10/25 21:00 | CD Quintanar del Rey | UD Ibiza Eivissa | 5-4a.TAB |
| 29/10/25 21:00 | Real Jaén C.F. | CD Eldense | 1-3 |
| 29/10/25 21:00 | CF Lucena | Villarreal CF | 0-6 |
| 29/10/25 21:00 | CD Yuncos | Rayo Vallecano | 1-6 |
| 30/10/25 19:00 | Orihuela CF | UD Levante | --- |
| 30/10/25 19:00 | Puerto de Vega CF | Celta Vigo | --- |
| 30/10/25 19:30 | Valle de Egues | Andorre CF | --- |
| 30/10/25 20:00 | Atletico Baleares | Gimnastic Tarragone | --- |
| 30/10/25 20:00 | UD Logroñés | SD Ponferradina | --- |
| 30/10/25 20:00 | Real Ávila CF | Real Avilés | --- |
| 30/10/25 20:00 | Arenas Club de Getxo | Deportivo Alaves | --- |
| 30/10/25 21:00 | Real Murcia C. F. | Antequera CF | --- |
| 30/10/25 21:00 | CD Estepona | FC Malaga | --- |
| 30/10/25 21:00 | CA Antoniano | CD Castellon | --- |
| 30/10/25 21:00 | UD Samano | Deportivo La Coruna | --- |
| 30/10/25 21:00 | Club Lleida Esportiu | Espanyol Barcelone | --- |
| 30/10/25 21:00 | Atletico Palma del Rio | Bétis Séville | --- |